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<t>PLCγ/PKC</t> Pathway Protein and Gene Expression. (A) Immunoblot of PLCγ, p-PLCγ, PKC, p-PKC Proteins Expression in Testicular Tissues of Mice in Each Group; (B–E) Average Optical Densities of PLCγ, p-PLCγ, PKC and p-PKC Proteins Compared With That of β-Actin Protein, Respectively; (F&G) The mRNA Levels of PLCγ and PKC * p < .05 versus control group. # p < .05 versus model group. Data represent mean ± SD , n = 3. && p < .01 versus WZYZW low-dose group. $$ p < .01 versus WZYZW medium-dose group.
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PLCγ/PKC Pathway Protein and Gene Expression. (A) Immunoblot of PLCγ, p-PLCγ, PKC, p-PKC Proteins Expression in Testicular Tissues of Mice in Each Group; (B–E) Average Optical Densities of PLCγ, p-PLCγ, PKC and p-PKC Proteins Compared With That of β-Actin Protein, Respectively; (F&G) The mRNA Levels of PLCγ and PKC * p < .05 versus control group. # p < .05 versus model group. Data represent mean ± SD , n = 3. && p < .01 versus WZYZW low-dose group. $$ p < .01 versus WZYZW medium-dose group.

Journal: American Journal of Men's Health

Article Title: Wu Zi Yan Zong Wan Improves Triptolide-Induced Testicular Spermatogenic Dysfunction Through the Spermatogenic Microenvironment and the PLCγ/PKC Pathway

doi: 10.1177/15579883251371990

Figure Lengend Snippet: PLCγ/PKC Pathway Protein and Gene Expression. (A) Immunoblot of PLCγ, p-PLCγ, PKC, p-PKC Proteins Expression in Testicular Tissues of Mice in Each Group; (B–E) Average Optical Densities of PLCγ, p-PLCγ, PKC and p-PKC Proteins Compared With That of β-Actin Protein, Respectively; (F&G) The mRNA Levels of PLCγ and PKC * p < .05 versus control group. # p < .05 versus model group. Data represent mean ± SD , n = 3. && p < .01 versus WZYZW low-dose group. $$ p < .01 versus WZYZW medium-dose group.

Article Snippet: PLCγ (1:2000; Cell Signaling Technology Cat. No. 5385, RRID: AB_2797610, Rabbit monoclonal antibody, Boston, MA, USA), p-PLCγ (1:2000; Cell Signaling Technology Cat. No. 2821, RRID: AB_2798485, Rabbit monoclonal antibody, Boston, MA, USA).

Techniques: Gene Expression, Western Blot, Expressing, Control